LIGER (installed as rliger ) is a package for integrating and analyzing multiple single-cell datasets, developed by the Macosko lab and maintained/extended by the Welch lab. It relies on integrative non-negative matrix factorization to identify shared and dataset-specific factors.
Check out our Cell paper for a more complete description of the methods and analyses. To access data used in our SN and BNST analyses, visit our study on the Single Cell Portal.
LIGER can be used to compare and contrast experimental datasets in a variety of contexts, for instance:
Once multiple datasets are integrated, the package provides functionality for further data exploration, analysis, and visualization. Users can:
We have also designed LIGER to interface with existing single-cell analysis packages, including Seurat.
Consider filling out our feedback form to help us improve the functionality and accessibility of LIGER.
For usage examples and guided walkthroughs, check the vignettes directory of the repo.
NEW: Iterative Single-Cell Multi-Omic Integration Using Online iNMF
Jointly Defining Cell Types from Single-Cell RNA-seq and DNA Methylation
Running Liger directly on Seurat objects using Seurat wrappers
The rliger package requires only a standard computer with enough RAM to support the in-memory operations. For minimal performance, please make sure that the computer has at least about 2 GB of RAM. For optimal performance, we recommend a computer with the following specs:
The package development version is tested on Linux operating systems and Mac OSX.
The rliger package should be compatible with Windows, Mac, and Linux operating systems.
Before setting up the rliger package, users should have R version 3.4.0 or higher, and several packages set up from CRAN and other repositories. The user can check the dependencies in DESCRIPTION.
LIGER is written in R and is also available on the Comprehensive R Archive Network (CRAN). Note that the package name is rliger to avoid a naming conflict with an unrelated package. To install the version on CRAN, follow these instructions:
install.packages('rliger')To install the latest development version directly from GitHub, type the following commands instead of step 3:
install.packages('devtools')
library(devtools)
install_github('welch-lab/liger')Note that the GitHub version requires installing from source, which may involve additional installation steps on MacOS (see below).
Installation from CRAN is easy because pre-compiled binaries are available for Windows and MacOS. However, a few additional steps are required to install from source on MacOS/Windows (e.g. Install RcppArmadillo). (MacOS) Installing RcppArmadillo on R>=3.4 requires Clang >= 4 and gfortran-6.1. For newer versions of R (R>=3.5), it’s recommended to follow the instructions in this post. Follow the instructions below if you have R version 3.4.0-3.4.4.
sudo if needed):mv /path/to/clang4/ /usr/local/ .R/Makevars file containing following:# The following statements are required to use the clang4 binary
CC=/usr/local/clang4/bin/clang
CXX=/usr/local/clang4/bin/clang++
CXX11=/usr/local/clang4/bin/clang++
CXX14=/usr/local/clang4/bin/clang++
CXX17=/usr/local/clang4/bin/clang++
CXX1X=/usr/local/clang4/bin/clang++
LDFLAGS=-L/usr/local/clang4/libFor example, use the following Terminal commands:
cd ~
mkdir .R
cd .R
nano MakevarsPaste in the required text above and save with Ctrl-X.
The HDF5 library is required for implementing online learning in LIGER on data files in HDF5 format. It can be installed via one of the following commands:
| System | Command |
|---|---|
| OS X (using Homebrew or Conda) | brew install hdf5 or conda install -c anaconda hdf5 |
| Debian-based systems (including Ubuntu) | sudo apt-get install libhdf5-dev |
| Systems supporting yum and RPMs | sudo yum install hdf5-devel |
For Windows, the latest HDF5 1.12.0 is available at https://www.hdfgroup.org/downloads/hdf5/.
Note that the runUMAP function (which calls the uwot package) also scales to large datasets and does not require additional installation steps. However, using FIt-SNE is recommended for computational efficiency if you want to perform t-SNE on very large datasets. Installing and compiling the necessary software requires the use of git, FIt-SNE, and FFTW. For a basic overview of installation, visit this page.
Basic installation for most Unix machines can be achieved with the following commands after downloading the latest version of FFTW from here. In the fftw directory, run:
./configure
make
make install(Additional instructions if necessary). Then in desired directory:
git clone https://github.com/KlugerLab/FIt-SNE.git
cd FIt-SNE
g++ -std=c++11 -O3 src/sptree.cpp src/tsne.cpp src/nbodyfft.cpp -o bin/fast_tsne -pthread -lfftw3 -lm
pwdUse the output of pwd as the fitsne.path parameter in runTSNE.
Note that the above instructions require root access. To install into a specified folder (such as your home directory) on a server, use the --prefix option:
./configure --prefix=<install_dir>
make
make install
git clone https://github.com/KlugerLab/FIt-SNE.git
cd FIt-SNE
g++ -std=c++11 -O3 src/sptree.cpp src/tsne.cpp src/nbodyfft.cpp -I<install_dir>/include/ -L<install_dir>/lib/ -o bin/fast_tsne -pthread -lfftw3 -lm
pwdThe installation process of rliger should take less than 30 minutes.
The expected run time is 1 - 4 hours depending on dataset size and downstream analysis of the user’s choice.
The rliger package provides a small sample dataset for basic demos of the functions. You can find it in folder liger/tests/testdata/small_pbmc_data.RDS.
We also provide a set of scRNA-seq and scATAC-seq datasets for real-world style demos. These datasets are as follows:
GSM4138872_scRNA_BMMC_D1T1.RDS;GSM4138873_scRNA_BMMC_D1T2.RDS;GSM4138888_scATAC_BMMC_D5T1_peak_counts.RDS;GSM4138888_scATAC_BMMC_D5T1.RDS.interneurons_and_oligo.RDS;PBMC_control.RDS;PBMC_interferon-stimulated.RDS.Corresponding tutorials can be found in section Usage above.
This project is covered under the GNU General Public License 3.0.