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| TmCalculator-package | Melting Temperature of Nucleic Acid Sequences |
| c2s | convert a vector of characters into a string |
| check_filter | Check and filter valid base of nucleotide sequences |
| chem_correction | Correct melting temperature with chemical substances |
| complement | reverse complement and complement base of nucleotide sequences |
| GC | Calculate G and C content of nucleotide sequences |
| s2c | convert a string into a vector of characters |
| salt_correction | Calculate the corrected melting temperature with salt ions |
| TmCalculator | Melting Temperature of Nucleic Acid Sequences |
| Tm_GC | Calculate the melting temperature using empirical formulas based on GC content |
| Tm_NN | Calculate melting temperature using nearest neighbor thermodynamics |
| Tm_Wallace | Calculate the melting temperature using the 'Wallace rule' |